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26–28 Dec 2020
Asia/Shanghai timezone
2020金属组学研讨会暨北京金属组学平台年会及原子光谱沙龙年会顺利结束,期待再次相聚!

细菌Serratia Se1998介导铅纳米颗粒的生成机制研究

Not scheduled
15m
墙报

Speaker

Ms yinyin tang (CAS)

Description

**细菌Serratia Se1998介导铅纳米颗粒的生成机制研究** 唐寅寅1,2,刘丽红1,2,何滨1,2,胡立刚1,2*,江桂斌1,2 1中国科学院生态环境研究中心,环境化学与生态毒理学国家重点实验室,北京,100085 2中国科学院大学,北京,100049 E-mail: lghu@rcees.ac.cn 有毒重金属可以通过母岩风化或采矿、冶炼、燃料燃烧等人为活动在环境富集,通常很难从环境中去除。而微生物可迅速发展出对抗金属毒性的保护机制,通过氧化还原反应将高活性、高毒性难降解的金属离子钝化,来达到耐受有毒重金属的目的[1]。其中,微生物介导纳米颗粒的生成过程通常被看作一种内在的防御机制,而颗粒和蛋白的结合也起到稳定纳米颗粒的作用[2]。 本研究针对重金属铅展开,我们从陕西宝鸡铅锌尾矿中分离出一株对铅具有超富集能力的细菌Serratia Se1998[3],并在细菌Serratia Se1998 中发现铅主要结合在鞭毛蛋白上,在细菌Serratia Se1998 体内外发现了生物生成的硫化铅纳米颗粒。我们将此鞭毛蛋白基因序列敲除,得到一株新型细菌(knock out)。针对两种细菌进行铅暴露生长曲线测定,结果表明两种细菌在低于1mmol/L铅暴露量时生长趋势相同,毒性效应不明显。同时利用HPLC-ICP-MS联用系统对比两种细菌介导纳米颗粒生成占比,发现随着暴露浓度的降低,两种细菌介导生成纳米颗粒占比差别增大,同时说明鞭毛蛋白确实参与了纳米颗粒的生成过程。 参考文献 [1] Das S , Dash H R , Chakraborty J . Genetic basis and importance of metal resistant genes in bacteria for bioremediation of contaminated environments with toxic metal pollutants[J]. Applied Microbiology and Biotechnology, 2016, 100(7):1-18. [2] Raouf Hosseini M , Nasiri Sarvi M . Recent achievements in the microbial synthesis of semiconductor metal sulfide nanoparticles[J]. Materials Science in Semiconductor Processing, 2015, 40:293-301. [3] Chen BW, Fang LC, Yan XT, et al. A unique Pb-binding flagellin as an effective remediation tool for Pb contamination in aquatic environment[J]. Journal of Hazardous Materials. 2019,(363):34-40

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Ms yinyin tang (CAS)

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