Speaker
Prof.
婷 杨
(东北大学)
Description
金属在生命过程中扮演着重要的角色，研究金属在细胞内的形态转化、迁移及其与金属蛋白的相互作用对理解生命过程及特定疾病的发生发展具有重要意义。近些年来，我们在金属组学领域展开了相关研究工作，一方面为组学研究开发了一系列检测技术：(1)设计新型毛细管电泳借口与ICP-MS联用，研究了胞内中镉离子及纳米银的形态转化；(2)利用罗丹明B和锆金属有机骨架组成的元素-荧光双功能标签，对铜转运蛋白的迁移和重分布进行了示踪和双模式成像；(3) 研究了金属药物[Co(tpa)(cur)](ClO4)2在单细胞水平上的摄入和分布。另一方面，利用金属结合蛋白与特定金属的相互作用，以金属结合蛋白/多肽及噬菌体等为预处理媒介，建立了一系列样品前处理方法，实现了金属离子的选择性分离分析。
**参考文献**
1. Xue Men, Xing Wei, Xuan Zhang, Xiao-Yan Wang, Ming-Li Chen, Ting Yang, Jian-Hua Wang. Identification of intracellular cadmium transformation in HepG2 and MCF-7 cells. Talanta 2020, 218, 121065.
2. Qi-Xuan Sun, Xing Wei, Shang-Qing Zhang, Ming-Li Chen, Ting Yang, Jian-Hua Wang. Single cell analysis for elucidating cellular uptake and transport of cobalt curcumin complex with detection by time-resolved ICPMS. Analytica Chimica Acta 2019, 1066, 13-20.
3. Xiao-Yan Wang, Ting Yang, Xiao-Xiao Zhang, Ming-Li Chen, Jian-Hua Wang. In situ growth of gold nanoparticles on Hg2+-binding M13 phages for mercury sensing. Nanoscale 2017, 9(43), 16728-16734.
4. Ting Yang, Xiao-Yu Zhang, Xiao-Xiao Zhang, Ming-Li Chen, Jian-Hua Wang, Chromium(III) binding phage screening for the selective adsorption of Cr(III) and chromium speciation. ACS Applied Materials & Interfaces 2015, 7(38), 21287-21294.
5. Ting Yang, Jia-Wei Liu, Cui-Bo Gu, Ming-Li Chen, Jian-Hua Wang. Expression of arsenic regulatory protein in Escherichia coli for selective accumulation of methylated arsenic species. ACS Applied Materials and Interfaces 2013, 5 (7), 2767-2772.
6. Ting Yang, Ming-Li Chen, Lan-Hua Liu, Jian-Hua Wang, Purnendu K. Dasgupta. Iron(III) modification of Bacillus subtilis membranes provides record sorption capacity for arsenic and endows unusual selectivity for As(V), Environmental Science and Technology 2012, 46 (4), 2251-2256.
7. Ting Yang, Lan-Hua Liu, Jia-Wei Liu, Ming-Li Chen, Jian-Hua Wang. Cyanobacterium metallothionein decorated graphene oxide nanosheets for highly selective adsorption of ultra-trace cadmium. Journal of Materials Chemistry 2012, 22 (41), 21909-21916.
